In each case, tandem MS analysis showed neutral losses of fragments of 75 Da, 129 Da, and 307 Da, along with an m/z 308 ion, characteristic of glutathione conjugates (Supplementary Figure S1)
SAM levels were decreased, but S-adenosylhomocysteine (AdoHcy, SAH) levels were elevated considerably, with global DNA hypomethylation observed in both heterozygotes and homozygotes 38
[12, 13, 14] Although this approach has mainly been used in cancer immunotherapy to activate cytotoxic T lymphocytes (CTLs) against tumor antigens, several immunogenic peptide therapeutics have been developed as well for autoimmune diseases including SLE, [15,16] rheumatoid arthritis, [17,18] type 1 diabetes, [19,20] and multiple sclerosis, [21] with varying success
Louie did not reliably explain his proposed dose and duration thresholds
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